ABSTRACT
Objective To investigate the application value of combination of thinprep cytologic test (TCT),human papilloma virus (HPV),c-MYC and human chromosome telomerase gene (hTERC) genes to screen cervical cancer.Methods A total of 230 cases of the study objects was detected with TCT and HP,respectively.Amplification of c-MYC and hTERC genes was tested with fluorescence in situ hybridization (FISH) method.The histopathological results were the gold standard,and the sensitivity,specificity and accuracy of cervical intraepithelial neoplasia (CIN) Ⅱ / Ⅲ and squamous cell carcinoma (SCC) were compared with the four combined detection.Results Of 230 screened patients,there were 124 cases of TCT positive,155 cases of HPV positive,c-MYC gene amplified in 118 cases,and hTERC gene amplified in 128 cases.When TCT,HPV,c-MYC,and hTERC were used alone,the highest sensitivity was HPV (84.5％),the highest specificity was c-MYC (97.6％),and the highest accuracy was hTERC (85.2％).When the three indexes were used in coordination with each other,the sensitivity and accuracy of TCT + HPV + hTERC were the highest (98.6％ and 90.9％),and the specificity of TCT + c-MYC + hTERC was the highest (79.3％).When the four indexes were used together,the sensitivity was 98.6％,the specificity was 72.0％,and the accuracy was 89.1％.Conclusions Combined examination can improve the sensitivity and accuracy of screening cervical lesions,and the three combination of TCT + HPV + hTERC had the best effect,and c-MYC gene detection had the highest specificity.
ABSTRACT
Objective To investigate the application value of combination of thinprep cytologic test (TCT),human papilloma virus (HPV),c-MYC and human chromosome telomerase gene (hTERC) genes to screen cervical cancer.Methods A total of 230 cases of the study objects was detected with TCT and HP,respectively.Amplification of c-MYC and hTERC genes was tested with fluorescence in situ hybridization (FISH) method.The histopathological results were the gold standard,and the sensitivity,specificity and accuracy of cervical intraepithelial neoplasia (CIN) Ⅱ / Ⅲ and squamous cell carcinoma (SCC) were compared with the four combined detection.Results Of 230 screened patients,there were 124 cases of TCT positive,155 cases of HPV positive,c-MYC gene amplified in 118 cases,and hTERC gene amplified in 128 cases.When TCT,HPV,c-MYC,and hTERC were used alone,the highest sensitivity was HPV (84.5％),the highest specificity was c-MYC (97.6％),and the highest accuracy was hTERC (85.2％).When the three indexes were used in coordination with each other,the sensitivity and accuracy of TCT + HPV + hTERC were the highest (98.6％ and 90.9％),and the specificity of TCT + c-MYC + hTERC was the highest (79.3％).When the four indexes were used together,the sensitivity was 98.6％,the specificity was 72.0％,and the accuracy was 89.1％.Conclusions Combined examination can improve the sensitivity and accuracy of screening cervical lesions,and the three combination of TCT + HPV + hTERC had the best effect,and c-MYC gene detection had the highest specificity.
ABSTRACT
<p><b>OBJECTIVE</b>To study the contribution of genetic factors to the variance of serum leptin concentration in healthy, normotensive twins.</p><p><b>METHODS</b>A total of 57 pairs of twins were investigated: 28 female and 19 male pairs of monozygotic(MZ) twins, and 6 female and 4 male pairs of dizygotic(DZ) twins. The zygosity of twins was determined by comparing the concordance of the genotype of nine fluorescence-labeled microsatellite markers. The genetic analysis was performed using the variance-based method. Serum leptin levels were determined in duplicate by a radioimmunoassay Kit (Linco Research, Inc., St. Charles, Missouri) as previously described.</p><p><b>RESULTS</b>The test of genetic variance revealed a significantly larger within-pair variance of serum leptin in the DZ twins, in comparison with the MZ twins. The corresponding heritability for serum leptin was 8%. Adjusted for BMI, gender, and uric acid (UA), the heritability for serum leptin was 0.18%. Log leptin correlated significantly with blood pressure (SBp r=0.355 P<0.001; DBp r=0.339 P<0.001). Stepwise multiple linear regression analysis revealed that only BMI, gender and UA were linked independently to serum leptin levels(R(2)=0.788, P<0.001).</p><p><b>CONCLUSION</b>The above data indicate that environmental factors other than genetic factors are important determinants of leptinemia in normal subjects.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Body Mass Index , Genotype , Leptin , Blood , Genetics , Microsatellite Repeats , Genetics , Twins, Dizygotic , Genetics , Twins, Monozygotic , GeneticsABSTRACT
Objective To study on the efficiency of topical pharyngeal anesthesia in gastroscopy and the factors related to the tolerance of patients. Methods A randomized double-blind placebo-controlled study was conducted. Two hundred and three subjects consented to participate in this study underwent gastroscopy. Relative Risks (RR) of patients' discomfort in pharyngeal anesthesia were calculated, anxiety and other potential confounding factors by using logistic regression analysis. Results Compared with placebo controls, the RR of patient discomfort on intubation was 0.37 (95 % CI 0. 20-0. 70) , patients aged less than 40 years had RR higher than those of aged 40 or over ( RR 2. 13 , 95% CI 1. 09-4. 15 ) . With subgroup analysis in those patients less than 40 years old and undergoing gastroscopy for the first time, the RR of patients' discomfort was 0. 23 (95% CI 0. 07-0. 77) and 0. 24 (95% CI 0. 10-0. 58) for the topical anesthesia. Conclusion Topical pharyngeal anesthesia appears to be effective in diminishing the discomfort during endoscopy in patients less than 40 years old and those undergoing the procedure first time. Trait-anxiety is not a predictor of discomfort.